5 120601152054Aug. 2016 Update


 5 120601152054Aug. 2016 Update


Antibody humanization and optimization


Gene diagnosis and gene drug development


Treatment of colorectal cancer


Antibody humanize


Increase antibody selectivity and reduce the side effects 



5 120601152054Aug. 2016 Update



5 120601152054Aug. 2016 Update



5 120601152054Aug. 2016 Update



5 120601152054Aug. 2016 Update



2008~2016 Publications5 120601152054Aug. 2016 Update

2016 Publications

  1. Prijovich ZM, Burnouf PA, Chou HC, Huang PT, Chen KC,Cheng TL, Leu YL, Roffler SR.Synthesis and Antitumor Properties of BQC-Glucuronide, a Camptothecin Prodrug for Selective Tumor Activation.Mol Pharm. 2016 Apr 4;13(4):1242-50.
  2. Huang WC, Burnouf PA, Su YC, Chen BM, Chuang KH, Lee CW, Wei PK,Cheng TL*, Roffler SR*.Engineering Chimeric Receptors To Investigate the Size- and Rigidity-Dependent Interaction of PEGylated Nanoparticles with Cells.ACS Nano. 2016 Jan 26;10(1):648-62.


  1. Huang CC, Kuo KK, Cheng TC, Chuang CH, Kao CH, Hsieh YC, Cheng KH, Wang JY, Cheng CM, Chen CS,Cheng TL*.Development of Membrane-Bound GM-CSF and IL-18 as an Effective Tumor Vaccine.PLoS One. 2015 Jul 17;10(7):
  2. Cheng TC, Pan CH, Chen CS, Chuang KH, Chuang CH, Huang CC, Chu YY, Yang YC, Chu PY, Kao CH, Hsieh YC,Cheng TL*.Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies.Vet J. 2015 Jul;205(1):107-9.
  3. Tung HY, Su YC, Chen BM, Burnouf PA, Huang WC, Chuang KH, Yan YT,Cheng TL, Roffler SR.Selective Delivery of PEGylated Compounds to Tumor Cells by Anti-PEG Hybrid Antibodies.Mol Cancer Ther. 2015 Jun;14(6):1317-26.
  4. Cheng TC, Chuang KH, Roffler SR, Cheng KW, Leu YL, Chuang CH, Huang CC, Kao CH, Hsieh YC, Chang LS,Cheng TL*, Chen CS*. Discovery of specific inhibitors for intestinal E. coli  β-glucuronidase through in silico virtual screening.
  5. Hsieh YT, Chen KC, Cheng CM, Cheng TL, Tao MH, Roffler SR. Impediments to Enhancement of CPT-11 Anticancer Activity by E. coli Directed Beta-Glucuronidase Therapy.PLoS One. 2015 Feb 17;10(2):e0118028.
  6. Changchien JJ, Chen YJ, Huang CH, Cheng TL, Lin SR, Chang LS. Quinacrine induces apoptosis in human leukemia K562 cells via p38 MAPK-elicited BCL2 down-regulation and suppression of ERK/c-Jun-mediated BCL2L1 expression.Toxicol Appl Pharmacol. 2015 Apr 1;284(1):33-41

2014 Publications

  1. Su YC, Cheng TC, Leu YL, Roffler SR, Wang JY, Chuang CH, Kao CH, Chen KC, Wang HE, Cheng TL*.PET Imaging of β-Glucuronidase Activity by an Activity-Based 124I-Trapping Probe for the Personalized Glucuronide Prodrug Targeted Therapy.Mol Cancer Ther. 2014 Dec;13(12):2852-63. 2013 IF=6.107 (23/202=11.38 in ONCOLOGY.
  2. Chien-Han Kao, Jaw-Yuan Wang, Kuo-Hsiang Chuang, Chih-Hung Chuang, Ta-Chun Cheng, Yuan-Chin Hsieh, Yun-long Tseng, Bing-Mae Chen, Steve R. Roffler, Tian-Lu Cheng*. One-step mixing with humanized anti-mPEG bispecific antibody enhances tumor accumulation and therapeutic efficacy of mPEGylated nanoparticles.Biomaterials. 2014 Dec;35(37):9930-40. 2013IF=8.312 (2/77=2.5%) in ENGINEERING, BIOMEDICAL.
  3. Kuo-Hsiang Chuang,Chien-Han Kao,Steve R. Roffler,Ssu-Jung Lu,Ta-Chun Cheng, Yun-Ming Wang,Chih-Hung Chuang,Yuan-Chin Hsieh,Yeng-Tseng Wang,Jaw-Yuan Wang,Kuo-Yi Wengand Tian-Lu Cheng*. Development of an Anti-Methoxy Poly(ethylene glycol) (α-mPEG) Cell-Based Capture System to Measure mPEG and mPEGylated Molecules. Macromolecules.2014, 47, 6880−6888.2013 IF=5.927 (3/82, 3.7%) in POLYMER SCIENCE.
  4. Kuo-Hsiang Chuang, Yuan-Chin Hsieh, I-Shiuan Chiang, Chih-Hung Chuang, Chien-Han Kao, Ta-Chun Cheng, Yeng-Tseng Wang, Wen-Wei Lin, Bing-Mae Chen, Steve R. Roffler, Ming-Yii Huang, Tian-Lu Cheng*. High-Throughput Sorting of the Highest Producing Cell via a Transiently Protein-Anchored System.PLoS One. 2014 Jul 18;9(7):e102569. 2013 (8/55=14.5% inMULTIDISCIPLINARY SCIENCES
  5. Cheng CM, Tzou SC, Zhuang YH, Huang CC, Kao CH, Liao KW, Cheng TC, Chuang CH, Hsieh YC, Tai MH, Cheng TL*.FunctionalproductionPLoS One. 2014 May 13;9(5):e97367.2013 (8/55=14.5% in MULTIDISCIPLINARY SCIENCES
  6. Chen YJ, Lin HC, Chen KC, Lin SR, Cheng TL, Chang LS. Taiwan cobra phospholipase A2 suppresses ERK-mediated ADAM17 maturation, thus reducing secreted TNF-α production in human leukemia U937 cells.Toxicon. 2014 May 27;86C:79-88.
  7. Su YC, Al-Qaisi TS, Tung HY, Cheng TL, Chuang KH, Chen BM, Roffler SR. Mimicking the germinal center reaction in hybridoma cells to isolate temperature-selective anti-PEG antibodies.MAbs. 2014 May 14;6(4).
  8. Chuang CH, Wang WJ, Li CF, Ko CY, Chou YH, Chuu CP, Cheng TL*, Wang JM*. The combination of the prodrugs perforin-CEBPD and perforin-granzyme B efficiently enhances the activation of caspase signaling and kills prostate cancer.Cell Death Dis. 2014 May 8;5:e1220.2013 IF=5.177 (49/185=26.5%) in CELL BIOLOGY.
  9. Chen YJ, Wu SC, Chen CY, Tzou SC, Cheng TL, Huang YF, Yuan SS, Wang YM. Peptide-based MRI contrast agent and near-infrared fluorescent probe for intratumoral legumain detection.

2013 publications

  1. Cheng TC, Chuang KH, Chen M, Wang HE, Tzou SC, Su YC, Chuang CH, Kao CH, Chen BM, Chang LS, Roffler SR, Cheng TL*.Sensitivity of PEGylated interferon detection by anti-polyethylene glycol (PEG) antibodies depends on PEG length.Bioconjug Chem.2013 Aug 21;24(8):1408-13.2013 IF=4.821 (7/58=12.1%) in CHEMISTRY, ORGANIC
  2. Liu WH, Chen YJ, Cheng TL, Lin SR, Chang LS.Cross talk between p38MAPK and ERK is mediated through MAPK-mediated protein phosphatase 2A catalytic subunit α and MAPK phosphatase-1 expression in human leukemia U937 cells.Cell Signal. 2013 Sep;25(9):1845-51
  3. Chen SH, Kuo YT, Cheng TL, Chen CY, Chiu YY, Lai JJ, Chang CC, Jaw TS, Wang YM, Liu GC.In vivo magnetic resonance imaging of mice liver tumors using a new gadolinium-based contrast agent.  Kaohsiung J Med Sci. 2013 May;29(5):246-53.
  4. Cheng CM, Chen FM, Lu YL, Tzou SC, Wang JY, Kao CH, Liao KW, Cheng TC, Chuang CH, Chen BM, Roffler S,Cheng TL*.Expression of β-glucuronidase on the surface of bacteria enhances activation of glucuronide prodrugs.Cancer Gene Ther. 2013 May;20(5):276-81. 2013 IF=2.553(58/165=35.2%) in BIOTECHNOLOGY & APPLIED MICROBIOLOGY.
  5. Wu SC, Lin KLWang TPTzou SCSingh GChen MHCheng TLChen CYLiu GCLee TWHu SHWang YM.Imaging specificity of MR-optical imaging agents following the masking of surface charge by poly(ethylene glycol). 2013 May;34(16):4118-27.
  6. Lu CY, Tsai HL, Uen YH, Hu HM, Chen CW, Cheng TL, Lin SR, Wang JY. Circulating tumor cells as a surrogate marker for determining clinical outcome to mFOLFOX chemotherapy in patients with stage III colon cancer.Br J Cancer. 2013 Mar 5;108(4):791-7.






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